A new method and tool for detection and quantification of PM oxidative potential

作者：Ciriello Francesco; Gualtieri Maurizio^*; Longhin Eleonora; Ruffo Riccardo; Camatini Marina; Parenti Paolo

来源：Environmental Science and Pollution Research, 2015, 22(16): 12469-12478.

DOI：10.1007/s11356-015-4551-2

• Particulate matter
• Redox cycling
• Cytochrome c
• TCEP
• Gold electrode

Airborne particulate matter (PM) contains several quinones, which are able to generate reactive oxygen species impacting on cell viability. A method able to detect and quantify PM oxidative potential, based on the cytochrome c (cyt-c) reduction by means of superoxide anion produced through quinones redox cycling in the presence of reducing agents, is here described. Tris(2-carboxyethyl) phosphine resulted to be the most efficient reducing agent among the ones tested. The procedure included rapid particles extraction, followed by two alternative analytical methods, a spectrophotometric assay based on the initial rate of cyt-c reduction at 550 nm, and an amperometric assay, based on self-assembled monolayers modified gold electrodes. The smallest amount of PM needed to obtain an evaluable signal is 2 mu g. The described procedure may represent a starting point to develop devices for PM measurements in polluted atmospheric environments.

• 出版日期2015-8