Lithocholic acid upregulates uPAR and cell invasiveness via MAPK and AP-1 signaling in colon cancer cells

作者：Baek Min Kyung; Park Jung Sun; Park Ji Hye; Kim Mi Ha; Kim Ho Dong; Bae Woo Kyun; Chung Ik Joo; Shin Boo Ahn; Do Jung Young^*

来源：Cancer Letters, 2010, 290(1): 123-128.

DOI：10.1016/j.canlet.2009.08.030

• LCA
• Erk-1/2
• AP-1
• uPAR
• Colon cancer

The secondary bile acid lithocholic acid (LCA) induced expression of urokinase-type plasminogen activator receptor (uPAR) and enhanced cell invasiveness in colon cancer cells. A dominant negative mutant or a specific inhibitor of MEK-1 suppressed LCA-induced uPAR expression. Deletions and site-directed mutagenesis revealed that the AP-1 site was required for LCA-induced uPAR transcription. LCA-mediated enhanced cell invasiveness was partially abrogated by uPAR neutralizing antibody and inhibitors of both Erk-1/2 and AP-1. These results suggest that LCA induces uPAR expression via Erk-1/2 and AP-1 pathway and, in turn, stimulate invasiveness of human colon cancer cells.

• 出版日期2010-4-1