Acute exercise increases hippocampal TNF-alpha, Caspase-3 and Caspase-7 expression in healthy young and older mice

作者：Packer N; Hoffman Goetz L^*

来源：Journal of Sports Medicine and Physical Fitness, 2015, 55(4): 368-376.

• Exercise
• Aging
• Cytokines
• Apoptosis
• Hippocampus

Aim. Regular exercise may protect against cognitive decline by preventing central inflammation. The effect of an acute exercise bout on central cytokine and apoptotic protein expression is not known. The brain may be protected from transient oxidative stress such as that induced by acute-exercise. The purpose of this exploratory study was to determine the effect of a single bout of intense exercise on hippocampal expression of inflammatory mediators (TNF-alpha) and apoptotic proteins (caspase-3, caspase-7), and to evaluate any potential age-related differences.
Methods. Using a C57BL/6 mouse model (N.=98), we evaluated the effect of an acute exercise bout (90 minutes of treadmill running: 10 min warm-up, 30 min at 22m.min(-1), 30 min at 25m.min(-1), and 30 min at 28m.min(-1), 2 degrees slope) on hippocampal inflammation in young (3-4 months), middle-aged (13-14 months) and older (16-17 months) C57BL/6 mice.
Results. Our results show post-exercise increases in hippocampal TNF-alpha and caspase-3/7 in each age group (main effect of acute exercise, P<0.05). Older mice displayed higher TNF-alpha (main effect of age, P<0.05) expression compared with younger animals at baseline. Young mice demonstrated greater increases in caspase-7 following acute exercise, compared to older mice (interaction effect for caspase-7, P<0.05).
Conclusion. Given the relationship between aging, inflammation and neurodegenerative disease, and the protective effects of exercise, we cautiously propose that acute-exercise induced inflammation may be a normal physiologic response that elicits a favorable (anti-inflammatory) hippocampal environment.

• 出版日期2015-4