Anticancer properties of low molecular weight oat beta-glucan - An in vitro study

作者：Choromanska Anna^*; Kulbacka Julita; Rembialkowska Nina; Pilat Justyna; Oledzki Remigiusz; Harasym Joanna; Saczko Jolanta

来源：International Journal of Biological Macromolecules, 2015, 80: 23-28.

DOI：10.1016/j.ijbiomac.2015.05.035

• Oat beta-glucan
• Melanoma
• Skin cancer

Anticancer properties of 1-3,1-4 oat beta glucan are under intensive investigation now. Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation. Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application. According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1. The low molecular weight beta-glucan from oat significantly deceased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly deceased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic. Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative. Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.

• 出版日期2015-9