Developmental Competence of Morphologically Poor Oocytes in Relation to Follicular Size and Oocyte Diameter in the Pig

作者：Kim Jinyoung; You Jinyoung; Hyun Sang Hwan; Lee Gene; Lim Jeongmook; Lee Eunsong^*

来源：Molecular Reproduction and Development, 2010, 77(4): 330-339.

DOI：10.1002/mrd.21148

The objective of this study was to assess the practical usefulness of morphologically poor oocytes (MPCOCs) in relation to follicular size and oocyte diameter Oocytes collected from medium (3-8 mm in diameter) and small (<3 mm) follicles were classified into five categories of morphologically good oocytes (MGCOCs) from medium follicles (MA, control), MPCOCs with larger and smaller diameters from medium follicles (ML and MS, respectively), and those from small follicles (SL and SS, respectively) The oocytes were examined for maturation and developmental competence after parthenogenesis and somatic cell nuclear transfer (SCNT) Nuclear maturation of ML oocytes (91%) was similar to that of control oocytes (94%), but higher than MS (80%), SL (79%), and SS (63%) oocytes This pattern was also observed in the intracellular glutathione level, p34(cdc2) kinase activity, and gene (CDK1, PCNA, and ERK2) expression levels in in vitro-matured oocytes ML oocytes showed a similar proportion of blastocyst formation (20%) after SCNT to control oocytes (21%) In addition, the use of ML oocytes resulted in a 50% farrowing rate with 18% efficiency of piglet production after SCNT embryo transfer, while control oocytes showed a 60% farrowing rate with 24% production efficiency Our results demonstrate that MPCOCs, if appropriately selected, have a comparable ability to MGCOCs in supporting not only in vitro blastocyst formation, but also development to term in vivo after SCNT These oocytes can be used as a source for in vitro production of embryos with normal in vivo viability in pigs.

• 出版日期2010-4