A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan

作者：Diao Qizhi; Li Yuan; Zhou Mi; Xie Guoming^*

来源：International Conference on Materials for environmental Protection and Energy Application (MEPEA 2011), 2011-09-27 to 2011-09-28.

DOI：10.4028/www.scientific.net/AMR.343-344.1207

• Immunosensor
• myeloperoxidase
• self-assemble
• multi- walled carbon nanotubes
• horseradish peroxidase

A new electrochemical immunosensor for scrum myeloperoxidase (MPO) has been developed based on the self-assembly multilays of multi-walled carbon nanotubes (MWNTs), thionine (THI), gold nanoparticles (GNPs) and chitosanon (CHIT) on the glassy carbon electrode (GCE). The antibody of MPO (anti-MPO) was absorbed on the surface of GNPs monolayer. Horseradish peroxidase (HRP) was employed to block non-specific binding and amplify the response current signal. It was observed that the peak current was linear with the MPO concentration in a range of 2.5-125 mu gl-l. The detection limit was 1.425 mu gl-l (S/N=3). Correlation analysis showed that this new immunosensor assay has a significant correlation with enzyme-linked immunosorbent assay (ELISA) (r=-0.96, p>0.05) for 40 clinical specimens.

• 出版日期2012
• 单位重庆医科大学