科研之友
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摘要
Background: Currently used techniques for diagnosing ischemic injury, such as magnetic resonance imaging and computed tomography, are not easily accessible for basic research using small animals due to their high cost and low availability. New method: We investigated the dynamic recovery of infarct regions in ischemia-injured brains using indocyanine green (ICG), which is inexpensive and readily available. This dye was used to visualize blood vessels and infarct area, and to measure blood flow after a photothrombotic ischemic operation (PIO). Mice were injected with ICG via the tail vein, and a time-series of fluorescence signal images was acquired before and after PIO. We then applied color codes to arteries and veins in the images and analyzed ICG intensity and dynamics. Results: These time-series stacked images showed changes in pial vessel morphology after PIO. Further, a map of maximum fluorescence intensity showed an infarct in the dorsal cortical region. Changes in the blood flow index and mean transit time were also observed in the infarct region after PIO. Comparison with existing method(s): Our application of ICG imaging provided a range of information on PIO-induced infarcts in mice with relative ease and in a cost-effective manner. Conclusion: Our results show that optical imaging using ICG combined with a time-series analysis of molecular dynamics can be a useful tool for the anatomical and physiological monitoring of cortical ischemia.
- 出版日期2015-6-15
