摘要

Near-infrared spectroscopy is a widely-used nondestructive analytical tool, but the disadvantages of weak band intensities and greatly overlapped bands have limited its application. In this review, several methods to overcome the drawbacks of NIR spectroscopy, such as second-derivative spectrum, two-dimensional correlation spectroscopy and chemometrics, are introduced. The applications of NIR spectroscopy in the studies of the structures and contents of both proteins and polymers with amide groups are discussed. All these show that the methods help a lot of in the qualitative and quantitative analysis of near-infrared spectroscopy and broaden the potential application of NIR spectroscopy.