Adenovirus-mediated overexpression FADD induces a significant antitumor effect on human colorectal cancer cells both in vitro and in vivo

作者：He, Xueling; Peng, Xu; Liu, Yan; Zhang, Xiaomei; Li, Hualing; Yin, Hailin^*

来源：Cellular and Molecular Biology, 2018, 64(6): 31-35.

DOI：10.14715/cmb/2018.64.6.6

• Wnt/beta-catenin
• Fas-associated death domain protein (FADD)
• Apoptosis
• Colon cancer

The Wnt/beta-catenin signaling pathway plays important roles in cancers such as colorectal cancer. Colon cancer cells secrete and express high levels of beta-catenin, which may stimulate autocrine signaling and further enhance activities of the canonical Wnt signaling pathway. Free beta-catenin in the cytoplasm and nucleus leads to its association with T cell factor (TCF)/lymphocyte enhancing factor (Let) transcription factors, and subsequent transcriptional activation of downstream target genes. FADD plays a key role in cellular apoptosis in many different types of cancer. Therefore, a recombinant adenovirus is constructed, in which an apoptosis gene FADD is placed under control of a promoter containing Tef-responsive elements. It is observed that FADD overexpression can suppress cell growth and enhance apoptosis of SW480 cells in vitro. In addition, Ad-FADD can also suppress the growth of subcutaneous xenografts in the nude mice. Together, these results suggest that Ad-FADD has anti-proliferative and pro-apoptotic effects in colon cancer cells, which provides a novel strategy for treatment of colorectal cancer.

• 出版日期2018
• 单位四川大学