A multidisciplinary approach to diagnose naturally occurring bovine tuberculosis in Brazil

作者：Marassi Carla D^*; Medeiros Luciana; Figueiredo Eduardo; Fonseca Leila S; Duarte Rafael; Paschoalin Vania; Oelemann Walter M R; Lilenbaum Walter

来源：Pesquisa Veterinaria Brasileira, 2013, 33(1): 15-20.

DOI：10.1590/S0100-736X2013000100004

• Tuberculosis
• serological
• ELISA
• MPB70
• MPB83
• IFN
• cattle

Marassi C. D., Medeiros L., Figueiredo E., Fonseca L. S., Duarte R., Paschoalin V., Oelemann W. M. R. %26 Lilenbaum W. 2103. A multidisciplinary approach to diagnose naturally occurring bovine tuberculosis in Brazil. Pesquisa Veterinaria Brasileira 33(1): 15-20. Laboratorio de Bacteriologia Veterinaria, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Rua Prof. Hernani de Mello 101, Lab. 309, Niteroi, RJ 24210-130, Brazil. E-mail: [e-mail] %26lt;br%26gt;A herd infected naturally with tuberculosis was investigated by different diagnostic methods. Ninety days after a screening test that identified 21 cows as skin test positive, a Comparative Intradermal Tuberculin Test (CITT) was performed in those 21 cows and in 29 other randomly selected skin test negative cows. Milk samples and nasal swabs were collected prior to the CITT for bacteriological culture and PCR, while blood samples were collected for IFN release and antibody responses to MPB70 and MPB83, at three time points post tuberculin injection. Animals positive by CITT were slaughtered and disease confirmation undertaken. Based on the Kappa test, IFN was comparable to the standard tests (culture, PCR and CITT) at all three sampling points. Results from both antibody ELISAs were similar but were not comparable to the standard tests. T-test analysis of the CITT, IFN and ELISAs demonstrated that their performances were not correlated. There is increasing recognition that individually, available diagnostic tests do not detect all infected cattle. Therefore, a comprehensive strategy for the diagnosis of bovine TB should include test results for the detection of both cellular and humoral immune responses where there may be animals at different stages of infection.

• 出版日期2013-1