A MULTICHANNELED BACTERIAL CELLULOSE SCAFFOLD FOR 3D IN VITRO CANCER CULTURE

作者：Luo, Honglin; Gu, Peng; Xiong, Guangyao; Hu, Da; Zhu, Yong; Wan, Yizao^*

来源：Cellulose Chemistry and Technology, 2016, 50(1): 49-56.

• bacterial cellulose
• multichannel
• pore structure
• tumor model
• cancer cell

Artificial three-dimensional (3D) in vitro tumor models mimicking the native cell architecture and environments are highly desirable tools for studying tumor progression and screening therapeutics. In this work, a 3D bacterial cellulose (BC) scaffold with multichanneled macropores (similar to 300 mu m) was fabricated. The obtained MM-BC scaffold was characterized by SEM, mercury intrusion porosimeter, contact angle and mechanical measurements, and determined for its potential as a tumor model. It was demonstrated that the MM-BC scaffold exhibited hierarchical pore structure and sufficient mechanical strength. Moreover, the MM-BC scaffold supported the adhesion, migration, and proliferation of primary culture cancer cells, allowed the cells' infiltration into the core of the scaffold. The results suggested that the MM-BC scaffold can be an effective in vitro tumor model to study cancer progression and drug screening.

• 出版日期2016-1
• 单位天津大学; 天津医科大学