An improved fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran: its origin and application

作者：Moriya Takashi; Kawamata Ayano; Takahashi Yusuke; Iwabuchi Yoshiharu; Kanoh Naoki^*

来源：Chemical Communications, 2013, 49(98): 11500-11502.

DOI：10.1039/c3cc47264g

We have developed a fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran. The reaction of NAD(P)(+) with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)(+). This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.

• 出版日期2013