Lycopene protects against LPS-induced proinflammatory cytokine cascade in HUVECs

作者：Wang, Yunhui; Gao, Yanfei; Yu, Wentao; Jiang, Zhie; Qu, Jinlong; Li, Kunxia^*

来源：Pharmazie, 2013, 68(8): 681-684.

DOI：10.1691/ph.2013.3507

• REGULATORY T-CELLS
• NF-KAPPA-B
• ENDOTHELIAL-CELLS
• ATHEROSCLEROTIC PLAQUE
• EXPRESSION
• ACTIVATION
• ADHESION
• DISEASE
• MICE

The aim of the study was to explore whether lycopene protects against the activation of human umbilical vein endothelial cells (HUVECs) induced by a proinflammatory stimulus. HUVECs were pretreated with different concentrations of lycopene (1 mu m or 10 mu m), then incubated with 1 mu g/ml LPS for 24h. After an incubation, the mRNA and protein levels of proinflammatory cytokines (MCP-1, IL-6, VCAM-1), the expression KLF2, TLR4, ERK1/2 and NF-kappa B were assayed. The result showed that lycopene treatment significantly suppressed the response of HUVECs to LPS and reduced the levels of proinflammatory cytokines. Also, lycopene increased KLF2 expression, while it inhibited the activation TLR4 and its downstream ERK and the NF-kappa B signaling pathway in HUVECs.

• 出版日期2013-8
• 单位烟台毓璜顶医院