A New Phosphorimetry for the Determination of Trace Alkaline Phosphatase Using Multi-wall Carbon Nanotubes and Its Diagnosis of Human Diseases

作者：Liu, Jia-Ming^*; Rao, Ying; Chen, Li; Wang, Xin-Xing; Lin, Li-Ping; Lin, Chang-Qing; Zhang, Li-Hong; Ou-Yang, Ying

来源：Journal of Fluorescence, 2010, 20(4): 933-941.

DOI：10.1007/s10895-010-0639-8

• Multi-wall carbon nanotubes
• Alkaline phosphatase
• Solid substrate-room temperature phosphorimetry
• Diagnosis of human diseases
• Signal magnification

Multi-wall carbon nanotubes (MWNTs) could be modified as water soluble MWNTs (it was called as MWNTs-A and MWNTs-B) by chemical methods. MWNTs-A and MWNTs-B could emit room temperature phosphorescence (RTP) signal on the surface of nitrocellulose membrane (NCM). A new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace alkaline phosphatase (ALP) was established based on the signal magnification effect of tween-80 and ALP on MWNTs-B's RTP intensity and the linear relationship between the content of ALP and the Delta I-P of the system. The linear range of this method was 0.0020-0.80 (fg spot(-1), sample volume: 0.40 mu l spot(-1)), the regression equation of working curve was Delta I-P = 0.8170 + 96.84m(ALP) (fg spot(-1)) (fg spot(-1)), correlation coefficient (r) was 0.9986. This method had high sensitivity (detection limit (LD): 1.4 ag spot(-1)), good selectivity (Er <=+/- 5%, coexistence species were of no interference), high precision (RSDs were 4.4%-1.2%) and accuracy. It was applied to the determination of trace ALP in human serum and the diagnosis of human diseases. The results were tallied with those of enzyme-linked immunosorbent assay (ELISA). The mechanism of new SS-RTP for the determination of trace ALP was discussed, which laid the theory foundation for the analytical application of MWNTs in life science.

• 出版日期2010-7
• 单位闽南师范大学; 漳州职业技术学院; 复旦大学