Acid, basic, and neutral peptidases present different profiles in chromophobe renal cell carcinoma and in oncocytoma

作者：Blanco Lorena; Larrinaga Gorka; Perez Itxaro; Lopez Jose I; Gil Javier; Agirregoitia Ekaitz; Varona Adolfo^*

来源：American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 2008, 294(4): F850-F858.

DOI：10.1152/ajprenal.00469.2007

• peptide signaling
• renal tumor behavior
• chromophobe carcinoma
• renal oncocytoma

Renal cell carcinomas (RCCs) are neoplasias with high prevalence and mortality. We previously reported that several peptidases may be involved in the pathophysiology of clear cell renal cell carcinoma (CCRCC). Now, to gain insight into the reasons that lead the various RCC types to behave very differently with regard to aggressiveness and response to anticancer treatments, we analyzed subsets of chromophobe renal cell carcinoma (ChRCC), and renal oncocytoma (RO), a benign tumor; as well as different grades and stages of CCRCCs. Particulate APN, APB, and APA activities were decreased in both ChRCC and RO (tumor vs. nontumor tissues). Interestingly, activities were downregulated in a tumor-type specific way and the intensities of the decreases were stronger in the benign tumor than in the malignant type. Moreover, when two key histopathological parameters for tumor prognosis (high vs. low stage and grade) were analyzed, increases of activity were also observed in several of these cell surface peptidases (APN, APB). Some soluble activities (APB, Asp-AP) were also downregulated in the RCCs. With respect to genetic expression, PSA and APN were in a positive correlation related to their activities in both ChRCC and RO; but not APB, Asp-AP, APA, and PGI. These results may suggest an involvement of several peptidases in the pathophysiology of renal cancer, since they presented different patterns of activity and expression in tumors with different behaviors.

• 出版日期2008-4