A Novel Nanocomposite Matrix Based on Silylated Chitosan and Multiwall Carban Nanotubes for the Immobilization of Urease

作者：Tiwari Ashutosh^*

来源：Journal of Inorganic and Organometallic Polymers and Materials, 2009, 19(3): 361-366.

DOI：10.1007/s10904-009-9280-x

• Chitosan
• Multiwall carbon nanotubes
• Nanocomposite matrix
• Covalent immobilization
• Urease

A nanocomposite matrix made up of silylated chitosan and multiwall carbon nanotubes (CHIT-SiO(2)-MWCNTs) was fabricated to investigate the immobilization of urease (Urs). Urs enzyme was covalently immobilized with the CHIT-SiO(2)-MWCNTs matrix using glutaraldehyde as a linker. The resulting Urs/CHIT-SiO(2)-MWCNTs biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) taking CHIT-SiO(2)-MWNTs as a reference. The influence of various parameters on Urs enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the Urs enzyme were calculated to be 0.51 mM and 89.02 mg/cm(2), respectively; indicating CHIT-SiO(2)-MWCNTs nanocomposite matrix has a high affinity to immobilize Urs enzyme.

• 出版日期2009-9