Adiponectin, a downstream target gene of peroxisome proliferator-activated receptor gamma, controls hepatitis B virus replication

作者：Yoon Sarah; Jung Jaesung; Kim Taeyeung; Park Sun; Chwae Yong Joon; Shin Ho Icon; Kim Kyongmin^*

来源：Virology, 2011, 409(2): 290-298.

DOI：10.1016/j.virol.2010.10.024

• Hepatitis B virus replication
• Adiponectin
• PPAR gamma

In this study, HepG2-hepatitis B virus (HBV)-stable cells that did not overexpress HBx and HBx-deficient mutant-transfected cells were analyzed for their expression of HBV-induced, upregulated adipogenic and lipogenic genes. The mRNAs of CCAAT enhancer binding protein alpha (C/EBP alpha), peroxisome proliferator-activated receptor gamma (PPAR gamma), adiponectin, liver X receptor alpha (LXR alpha), sterol regulatory element binding protein 1c (SREBP1c), and fatty acid synthase (FAS) were expressed at higher levels in HepG2-HBV and lamivudine-treated stable cells and HBx-deficient mutant-transfected cells than in the HepG2 cells. Lamivudine treatment reduced the mRNA levels of PPAR gamma and C/EBP alpha. Conversely, HBV replication was upregulated by adiponectin and PPAR gamma agonist rosiglitazone treatments and was downregulated by adiponectin siRNAs. Collectively, our results demonstrate that HBV replication and/or protein expression, even in the absence of HBx, upregulated adipogenic or lipogenic genes, and that the control of adiponectin might prove useful as a therapeutic modality for the treatment of chronic hepatitis B.

• 出版日期2011-1-20