Anti-differentiation non-coding RNA, ANCR, is differentially expressed in different types of brain tumors

作者：Malakootian Mahshid; Azad Fatemeh Mirzadeh; Fouani Youssef; Bajgan Elham Taheri; Saberi Hooshang; Mowla Seyed Javad^*

来源：Journal of Neuro-Oncology, 2018, 138(2): 261-270.

DOI：10.1007/s11060-018-2809-5

• ANCR
• Brain tumors
• LncRNAs
• Tumor marker

Long non-coding RNAs (lncRNAs) are important modulators of various cellular and molecular events, including cancer-associated pathways. The Anti-differentiation ncRNA (ANCR) is a key regulator of keratinocyte differentiation, where its expression is necessary to maintain epidermal progenitor's cells. Herein, we investigated the expression pattern of ANCR in the course of neural differentiation. Moreover, we used published RNAseq data and clinical samples to evaluate the alteration of ANCR expression in different cell types and brain tumors. Furthermore, we manipulated ANCR expression in glioma cell lines to clarify a potential functional role for ANCR in tumorigenesis. Our qRT-PCR results revealed a significant upregulation of ANCR in more malignant and less differentiated types of brain tumors (P = 0.03). This data was in accordance with down regulation of ANCR during neural differentiation. ANCR suppression caused an elevation in apoptosis rate, as well as a G1 cell cycle arrest in glioblastoma cell line. Altogether, our data demonstrated that ANCR may play a role in glioma genesis and that it could be considered as a potential diagnostic and therapeutic target to combat brain cancers.

• 出版日期2018-6