Manipulating motions of targeted single cells in solution by an integrated double-ring magnetic tweezers imaging microscope

作者：Wu Meiling; Yadav Rajeev; Pal Nibedita; Lu H Peter^*

来源：Review of Scientific Instruments, 2017, 88(7): 073703.

DOI：10.1063/1.4995362

Controlling and manipulating living cell motions in solution hold a high promise in developing new biotechnology and biological science. Here, we developed a magnetic tweezers device that employs a combination of two permanent magnets in up-down double-ring configuration axially fitting with a microscopic objective, allowing a picoNewton (pN) bidirectional force and motion control on the sample beyond a single upward pulling direction. The experimental force calibration and magnetic field simulation using finite element method magnetics demonstrate that the designed magnetic tweezers covers a linear-combined pN force with positive-negative polarization changes in a tenability of sub-pN scale, which can be utilized to further achieve motion manipulation by shifting the force balance. We demonstrate an application of the up-down double-ring magnetic tweezers for single cell manipulation, showing that the cells with internalized paramagnetic beads can be selectively picked up and guided in a controlled fine motion. Published by AIP Publishing.

• 出版日期2017-7