科研之友
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摘要
BACKGROUND: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by defects in insulin secretion and action, which can lead to damaged blood vessels and nerves. With respect to effective therapeutic approaches to treatment of DM, much effort has being made to investigate potential inhibitors against alpha-glucosidase and alpha-amylase from natural products. The edible marine brown alga Ecklonia cava has been reported to possess various interesting bioactivities, which are studied here. RESULTS: In this study, five phloroglucinal derivatives were isolated from Ecklonia cava: fucodiphloroethol G (1), dieckol (2), 6,6'-bieckol (3), 7-phloroeckol (4) and phlorofucofuroeckol A (5); compounds 1, 3 and 4 were obtained from this genus for the first time and with higher yield. The structural elucidation of these derivatives was completely assigned by comprehensive analysis of nuclear magnetic spectral data. The anti-diabetic activities of these derivatives were also assessed using an enzymatic inhibitory assay against rat intestinal alpha-glucosidase and porcine pancreatic alpha-amylase. Most of these phlorotannins showed significant inhibitory activities in a dose-dependent manner, responding to both enzymes, especially compound 2, with the lowest IC(50) values at 10.8 mu mol L(-1) (alpha-glucosidase) and 124.9 mu mol L-1 (alpha-amylase), respectively. Further study of compound 2 revealed a non-competitive inhibitory activity against alpha-glucosidase using Lineweaver-Burk plots. CONCLUSION: These results suggested that Ecklonia cava can be used for nutritious, nutraceutical and functional foods in diabetes as well as for related symptoms. () 2009 Society of Chemical Industry
- 出版日期2009-7
- 单位吉林省中医药科学院
