科研之友
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摘要
Background Insulin resistance is an underlying feature of both type 2 diabetes and metabolic syndrome. Currently, it is unclear whether nuclear factor (NF)-kappa B inducing kinase (NIK) plays a role in the development of insulin resistance. The present in vivo study investigated the roles of NIK and I kappa B kinase alpha (IKK alpha) in obesity-induced insulin resistance using animal models. @@@ Methods NIK expression was evaluated by Western blotting in male Lee mice and C57BL/6J mice fed a high-fat diet (HFD) (45% fat). After mefformin and sulfasalazine treatment, NIK expression was investigated during the improvement of insulin resistance. @@@ Results NIK was increased by about 1-fold in the renal tissues of Lep(ob) mice and C57BL/6J mice fed a HFD for 12 weeks. After 1 and 3 weeks of high-fat feeding, we observed an almost 50% decrease in NIK and IKK alpha expression in the liver and renal tissues of C57BL/6J mice. NIK expression was significantly lower in the liver and renal tissues of HFD-fed mice that were treated with insulin sensitizers, mefformin and sulfasalazine. However, IKK alpha expression was increased after metformin treatment in both tissues. @@@ Conclusion These results suggest a possible role of NIK in the liver and renal tissues of insulin-resistant mice. Chin Med J 2011;124(22):3646-3651
- 出版日期2011-11-20
- 单位中山大学
