Autophagy Protein Rubicon Mediates Phagocytic NADPH Oxidase Activation in Response to Microbial Infection or TLR Stimulation

作者：Yang Chul Su; Lee Jong Soo; Rodgers Mary; Min Chan Ki; Lee June Yong; Kim Hee Jin; Lee Kwang Hoon; Kim Chul Joong; Oh Byungha; Zandi Ebrahim; Yue Zhenyu; Kramnik Igor; Liang Chengyu; Jung Jae U^*

来源：CELL HOST %26 MICROBE, 2012, 11(3): 264-276.

DOI：10.1016/j.chom.2012.01.018

Phagocytosis and autophagy are two important and related arms of the host%26apos;s first-line defense against microbial invasion. Rubicon is a RUN domain containing cysteine-rich protein that functions as part of a Beclin-1-Vps34-containing autophagy complex. We report that Rubicon is also an essential, positive regulator of the NADPH oxidase complex. Upon microbial infection or Toll-like-receptor 2 (TLR2) activation, Rubicon interacts with the p22phox subunit of the NADPH oxidase complex, facilitating its phagosomal trafficking to induce a burst of reactive oxygen species (ROS) and inflammatory cytokines. Consequently, ectopic expression or depletion of Rubicon profoundly affected ROS, inflammatory cytokine production, and subsequent antimicrobial activity. Rubicon%26apos;s actions in autophagy and in the NADPH oxidase complex are functionally and genetically separable, indicating that Rubicon functions in two ancient innate immune machineries, autophagy and phagocytosis, depending on the environmental stimulus. Rubicon may thus be pivotal to generating an optimal intracellular immune response against microbial infection.

• 出版日期2012-3-15