Assessment of the systemic distribution of a bioconjugated anti-Her2 magnetic nanoparticle in a breast cancer model by means of magnetic resonance imaging

作者：Huerta Nunez L F E; Cleva Villanueva Lopez G; Morales Guadarrama A; Soto S; Lopez J; Silva J G; Perez Vielma N; Sacristan E; Gudino Zayas Marco E; Gonzalez C A^*

来源：Journal of Nanoparticle Research, 2016, 18(9): 284.

DOI：10.1007/s11051-016-3594-8

• Breast cancer
• Magnetic nanoparticles
• Anti-Her2
• Systemic distribution
• Magnetic resonance imaging
• Nanomedicine

The aim of this study was to determine the systemic distribution of magnetic nanoparticles of 100 nm diameter (MNPs) coupled to a specific monoclonal antibody anti-Her2 in an experimental breast cancer (BC) model. The study was performed in two groups of Sprague-Dawley rats: control (n = 6) and BC chemically induced (n = 3). Bioconjugated "anti-Her2-MNPs" were intravenously administered, and magnetic resonance imaging (MRI) monitored its systemic distribution at seven times after administration. Non-heme iron presence associated with the location of the bioconjugated anti-Her2-MNPs in splenic, hepatic, cardiac and tumor tissues was detected by Perl's Prussian blue (PPB) stain. Optical density measurements were used to semiquantitatively determine the iron presence in tissues on the basis of a grayscale values integration of T1 and T2 MRI sequence images. The results indicated a delayed systemic distribution of MNPs in cancer compared to healthy conditions with a maximum concentration of MNPs in cancer tissue at 24 h post-infusion.

• 出版日期2016-9-22