A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells

作者：Tahara Yoshiro; Kaneko Takeshi; Toita Riki; Yoshiyama Chiharu; Kitaoka Takuya; Niidome Takuro; Katayama Yoshiki; Kamiya Noriho; Goto Masahiro^*

来源：Journal of Controlled Release, 2012, 161(3): 713-721.

DOI：10.1016/j.jconrel.2012.05.001

• Gene delivery
• Protein delivery
• Nanoparticles
• Emulsions
• Solid-in-oil nanodispersion

A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.

• 出版日期2012-8-10