An Ethanol Biosensor Based on Simple Immobilization of Alcohol Dehydrogenase on Fe3O4@Au Nanoparticles

作者：Samphao Anchalee^*; Kunpatee Kanjana; Prayoonpokarach Sanchai; Wittayakun Jatu****; Svorc Lubomir; Stankovic Dalibor M; Zagar Kristina; Ceh Miran; Kalcher Kurt

来源：Electroanalysis, 2015, 27(12): 2829-2837.

DOI：10.1002/elan.201500315

• Magnetic-gold nanoparticles
• ethanol biosensor
• carbon paste electrode
• ethanol determination
• alcohol dehydrogenase

An ethanol biosensor based on alcohol dehydrogenase (ADH) attached to Au seeds decorated on magnetic nanoparticles (Fe3O4@Au NPs) is presented. ADH was immobilized on Fe3O4@Au NPs, which were subsequently fixed by a magnet on a carbon paste electrode modified with 5% (m:m) MnO2. Optimum conditions for the amperometric determination of ethanol with the biosensor were as follows: working potential +0.1 V (vs. Ag/AgCl); supporting electrolyte: 0.1 M phosphate buffer solution at pH 6.8 containing 0.25 mM of the coenzyme (NAD+); working electrode: carbon paste with magnetically attached Fe3O4@Au NPs (0.012 mg . cm(-2) electrode area) with immobilized alcohol dehydrogenase (120 units per cm(2) of electrode area). Linearity between signal and concentration was found for the range from 0.1 to 2.0 M ethanol (r(2) = 0.995) with a detection limit of 0.07 M, a sensitivity of 0.02 mu A . mM(-1) . cm(-2), a reproducibility of 4.0% RSD, and a repeatability of 2.7% RSD. The results for the determination of ethanol in alcoholic beverages showed good agreement with gas chromatography (GC) with recovery of 96.0-108.8%.

• 出版日期2015-12