Apoptosis induced by ultraviolet radiation or actinomycin D in HeLaS3 cells

作者：Chang JH; Oh KS; Moon YS; Kim GC; Park HJ; Um KI^*

来源：Korean Journal of Genetics, 2003, 25(4): 301-307.

• Bcl-2
• caspase-2
• caspase-3
• apoptosis
• HeLaS3 cells
• DNA ladder pattern

Ultraviolet radiation (UV) or actinomycin D (AMD) can induce apoptosis in many cancer cells. Understanding the mechanism involved in UV or AMD-induced apoptosis may improve its therapeutic efficacy. The present study has been performed to elucidate apoptosis induced by UV or AMD in HeLa S-3 cells. Three assays were employed in this study: Morphological assessments of apoptotic cells, analysis of DNA electrophoresis and western blot analysis. The number of apoptotic cells or quantitative assay of fragmented DNA in this cell line treated with UV or AMD was increased at 3 or 12 hours, respectively. DNA ladder patterns in the cells treated with UV or AMD also appeared at 3 or 12 hours, respectively. The expression of Bcl-2 and Caspase-2 decreased, and then disappeared in the cells. Precursor of Caspase-3 was degraded, and 20 kDa cleavage products were detected These results suggest that UV or AMID induce apoptosis of HeLa S3 cells via Caspase-3-dependent fashion, and this apoptosis is related to disappearance of Bcl-2 and Caspase-2.

• 出版日期2003-12