FGF-2 suppresses expression of nephronectin via JNK and PI3K pathways

作者：Kato Tadashi; Yamada Atsushi; Ikehata Mikiko; Yoshida Yuko; Sasa Kiyohito; Morimura Naoko; Sakashita Akiko; Iijima Takehiko; Chikazu Daichi; Ogata Hiroaki; Kamijo Ryutaro

来源：FEBS Open Bio, 2018, 8(5): 836-842.

DOI：10.1002/2211-5463.12421

• fibroblast growth factor-2
• Jun N-terminal kinase
• MC3T3-E1
• nephronectin
• phosphoinositide-3 kinase

Nephronectin (Npnt), an extracellular matrix protein, is a ligand for integrin 81 and is involved in the development of various organs, such as the kidneys, bones, liver, and muscles. Previously, we found that Npnt expression was inhibited by various cytokines including transforming growth factor- (Tgf-) and oncostatin M (Osm). Fibroblast growth factor (Fgf)-2, otherwise known as basic Fgf, also plays important roles in skeletal development and postnatal osteogenesis. In this study, Npnt expression was found to be suppressed by Fgf-2 in MC3T3-E1 cells, an osteoblast-like cell line, in a dose- and time-dependent manners. Furthermore, Fgf-2-mediated NpntmRNA suppression was shown to involve the Jun N-terminal kinase (JNK) and phosphoinositide-3 kinase (PI3K) pathways. Together, our results suggest that FGF-2 suppresses Npnt gene expression via JNK and PI3K pathways.

• 出版日期2018-5