Astaxanthin Inhibits Proliferation and Induces Apoptosis of Human Hepatocellular Carcinoma Cells via Inhibition of Nf-Kb P65 and Wnt/B-Catenin in Vitro

作者：Li, Jingjing; Dai, Weiqi; Xia, Yujing; Chen, Kan; Li, Sainan; Liu, Tong; Zhang, Rong; Wang, Jianrong; Lu, Wenxia; Zhou, Yuqing; Yin, Qin; Abudumijiti, Huerxidan; Chen, Rongxia; Zheng, Yuanyuan; Wang, Fan; Lu, Jie; Zhou, Yingqun; Guo, Chuanyong

来源：Marine Drugs, 2015, 13(10): 6064-6081.

DOI：10.3390/md13106064

• hepatocellular carcinoma
• astaxanthin
• apoptosis

Hepatocellular carcinoma (HCC) is a malignant tumor that can cause systemic invasion; however, the exact etiology and molecular mechanism are unknown. Astaxanthin (ASX), a powerful antioxidant, has efficient anti-oxidant, anti-inflammatory, and other activities, and has great research prospects in cancer therapy. We selected the human hepatoma cell lines, LM3 and SMMC-7721, to study the anti-tumor effect and related mechanisms of ASX. The cell lines were treated with different concentrations of ASX, and its solvent DMSO as a control, for different time periods and the results were determined using CCK8, qRT-PCR, WB, apoptotic staining, and flow cytometry. ASX induced significant apoptosis of HCC cells, and its effect may have been caused by NF-B p65 and Wnt/-catenin down-regulation via negative activation of PI3K/Akt and ERK. Antitumor research on ASX has provided us with a potential therapy for patients with hepatomas.

• 出版日期2015-10
• 单位同济大学; 苏州大学; 南京医科大学