Brain Natriuretic Peptide is Not Reno-Protective during Renal Ischemia-Reperfusion Injury in the Rat

作者：Kim Tad; Harman P Kent; Lyons Robert; Gaskins Roger B III; Hobson Charles E; Evans Stephen M; Tribble Curtis G; Ejaz A Ahsan; Beaver Thomas M^*

来源：Journal of Surgical Research, 2010, 164(1): E13-E19.

DOI：10.1016/j.jss.2010.06.041

• kidney
• ischemia/reperfusion
• animal model
• inflammatory cells

Background. Acute kidney injury (AKI) occurs in 30% of patients undergoing complex cardiovascular surgery, and renal ischemia-reperfusion (I/R) injury is often a contributing factor. A recent meta-analysis observed that perioperative natriuretic peptide administration was associated with a reduction in AKI requiring dialysis in cardiovascular surgery patients. This study was designed to further clarify the potential reno-protective effect of brain natriuretic peptide (BNP) using an established rat model of renal I/R injury.
Methods. The study comprised three groups (n = 10 kidneys each): (1) control (no injury); (2) I/R injury (45 min of bilateral renal ischemia followed by 3 h of reperfusion); and (3) BNP (I/R injury plus rat-BNP pretreatment at 0.01 mg/kg/min). Glomerular filtration rate (GFR) and a biomarker of AKI, urinary neutrophil gelatinase-associated lipocalin (uNGAL), were measured at baseline and at 30 minute intervals post-ischemia. Groups were compared using two-way repeated measures analysis of variance (mean +/- SD, significance P < 0.05).
Results. Baseline GFR measurements for control, I/R, and BNP groups were 1.07 +/- 0.55, 0.88 +/- 0.51, and 1.03 +/- 0.59 mL/min (P = 0.90), respectively. Post-ischemia, GFR was significantly lower in I/R and BNP compared with controls at 30 min, 1.29 +/- 0.97, 0.08 +/- 0.04, and 0.06 +/- 0.05 mL/min (P < 0.01), and remained lower through 3 h, 1.79 +/- 0.44, 0.30 +/- 0.17, and 0.32 +/- 0.12 mL/min (P < 0.01). Comparing I/R to BNP groups, GFR did not differ significantly at any time point. There was no significant difference in uNGAL levels at 1 h (552 +/- 358 versus 516 +/- 259 ng/mL, P = 0.87) or 2 h (1073 +/- 589 versus 989 +/- 218 ng/mL, P = 0.79) between I/R and BNP.
Conclusions. BNP does not reduce the renal injury biomarker, urinary NGAL, or preserve GFR in acute renal ischemia-reperfusion injury.

• 出版日期2010-11