科研之友
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摘要
T. crispa is a well known traditional medicinal plant used in India, Indonesia, Philippines and Malaysia. To date, there are numerous studies on this plant emphasizing it's antioxidant, antidiabetic, antimalarial and cosmetic effects. However, limited informations are available regarding T. crispa cytotoxicity potential. The objectives of this study were to determine the cytotoxicity potential and antioxidant activity of different T. crispa crude extracts. Viability of cells was measured using the MTT assay on MCF-7, MDA-MB-231, HeLa and 3T3 normal fibroblast cells while antioxidant activity was determined by measuring total flavonoid content, total phenolic content and DPPH free radical scavenging activity. Results obtained showed that the cell viability decreased in a dose-dependent manner in all cancer cells for each extract. The lowest IC50 (33.75 +/- 4.65 mu g/ml) was observed in MCF-7 using the methanol extract. Further more, there were significant differences for total phenolic content (p<0.01, df=2, X-2=9.836) and flavonoid content (p<0.05, df=0.02, X-2= 7.20) between each extract with the methanol extract having the highest activity for both phenolic (255.33 +/- 10.79 mg GAE/g sample) and flavonoid content (9.53 +/- 0.50 mg QE/ g sample). The DPPH assay showed that the methanol extract had highest scavenging activity in a dose-dependent manner where the IC50 value was 12 mu g/ml. T. crispa has a dose-dependent antiproliferative activity against many types of cancer cells where the lowest IC50 was present in the methanol extract on MCF-7. Further more, methanol crude extract of T. crispa had higher total phenolic and flavonoid content and free radical scavenging activity compared to water extract and chloroform extract.
- 出版日期2011-3
- 单位河北医科大学
