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摘要
This experiment was to investigate the effect of dried rumen digesta (DRD) levels on rumen kinetic gas production, rumen ammonia-nitrogen (NH3-N) and digestibility of nutrients by using in vitro gas production techniques. The experimental design was a completely randomized design (CRD) and the dietary treatments were DRD supplementation at 0, 4, 8, 12, 16 and 20 mg with 0.5 g of roughage and concentrate ratio at 70:30. The gas production was recorded at 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h of incubation and was used for calculated of kinetics gas. The results revealed that crude protein, NDF and ADF in DRD were 19.4%, 42.4% and 20.9% of DM, respectively. The intercept value (a) for the different treatments representing gas production from soluble fractions, gas production from the insoluble fraction (b), potential extent of gas production (a+ b) were not significantly different among treatments (P > 0.05). However, gas production rate (c) and cumulative gas production (96 h of incubation) were significantly different between the supplemented groups and control (P< 0.05) and were highest when supplementation of DRD at 8 mg and the values were 0.09 ml/h and 132.5 ml/0.5 g substrate, respectively (P< 0.05). NH3-N concentrations were linearly increased when increasing levels of DRD and were highest with DRD supplementation at 20 mg (25.3 mg%). Moreover, in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) were highest when 8 mg of DRD was supplemented and the mean values were 63.8% and 65.2% DM, respectively. Based on this experiment, it could be concluded that supplementation of DRD at 8 mg could improve rumen fermentation, in vitro kinetics gas, NH3-N concentration and digestibility.
- 出版日期2013-5
