Aged mice receiving caffeine since adulthood show distinct patterns of anxiety-related behavior

作者:Botton Paulo Henrique S; Pochmann Daniela; Rocha Andreia S; Nunes Femanda; Almeida Amanda S; Marques Daniela M; Porciuncula Lisiane O
来源:PHYSIOLOGY %26 BEHAVIOR, 2017, 170: 47-53.
DOI:10.1016/j.physbeh.2016.11.030

摘要

Caffeine is the psyc.hostimulant most consumed worldwide. Anxiogenic effects of caffeine have been described in adult animals with controversial findings about its anxiogenic potential. Besides, the effects of caffeine on anxiety with aging are still poorly known. In this study, adult mice (6 months old) started to receive caffeine (0.3 and 1.0 mg/mL, drinking water) during 12-14 months only in the light cycle and at weekdays. The open field (OF) and elevated plus maze (EPM) testing were used to determine the effects of caffeine on anxiety-related behavior in adult and aged mice (18-20 months old). Because aging alters synaptic proteins, we also evaluated SNAP-25 (as a nerve terminals marker), GFAP (as an astrocyte marker) and adenosine A(1) and A(2)A receptors levels in the cortex. According to the OF analysis, caffeine did not change both hypolocomotion and anxiety with aging. However, aged mice showed less anxiety behavior in the EPM, but after receiving Caffeine (0.3 mg/mL) during adulthood they were anxious as adult mice. While SNAP-25 and adenosine A2A receptors increased with aging, both GFAP and adenosine A(1) receptors were not affected. Caffeine at moderate dose prevented the age-related increase of the SNAP-25, with no effect on adenosine A(2A) receptors. The absence of effect for the highest dose suggests that tolerance to caffeine may have developed overtime. Aged mice showed high responsiveness to the OF, being difficult to achieve any effect of caffeine. On the other hand this substance sustained the adult anxious behavior over time in a less stressful paradigm, and this effect was coincident with changes in the SNAP-25, suggesting the involvement of this synaptic protein in the ability of caffeine to preserve changes related to emotionality with aging.

  • 出版日期2017-3-1