科研之友
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摘要
A Gram-stain negative, orange-pigmented, aerobic, non-motile and ovoid-to rod-shaped bacterial strain, designated CDA4(T), was isolated from a sediment sample collected from the sedimentation basin of a mariculture farm in Zhejiang province, China. The temperature range for growth of strain CDA4(T) was 15-40 degrees C, wirrth an optimum at 35 degrees C. The pH range for growth was 6.0-8.5, with an optimum around pH 7.5. NaCl was required for growth at the concentration range 0.5-5.0% (w/v), with an optimum at 2.0% (w/v). Chemotaxonomic analysis indicated that the main respiratory quinone was menaquinone 6 (MK-6), the predominant cellular fatty acids were iso-C-15:0, iso-C-15:1 G, unknown ECL 13.565 and iso-C-17:0 3-OH, and the major polar lipids were phosphatidylethanolamine, two unidentified lipids, two unidentified phospholipids and four unidentified aminolipids. The genomic DNA G+C content of strain CDA4(T) was 38.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CDA4(T) formed a distinct lineage in the clade of the family Flavobacteriaceae. Based on the polyphasic taxonomic characterization, strain CDA4(T) is considered to represent a novel species of a new genus, for which the name Maripseudobacter aurantiacus gen. nov., sp. nov. is proposed. The type strain of the type species is CDA4(T) (=KCTC 52409(T)=MCCC 1K03210(T)).
