Adhesin-Specific Nanomechanical Cantilever Biosensors for Detection of Microorganisms

作者：Tzen Tzuen Rong J^*; Cheng Yunyan R; Saeidpourazar Reza; Aphale Siddharth S; Jalili Nader

来源：Journal of Heat Transfer-Transactions of the ASME, 2011, 133(1): 011012.

DOI：10.1115/1.4002363

Lectins (adhesins) on bacterial surfaces play important roles in infection by mediating bacterial adherence to host cell surfaces via their cognate receptors. We have explored the use of alpha-D-mannose receptors as capturing agents for the detection of Escherichia coli using a microcantilever and have demonstrated that E. coli ORN178, which expresses normal type-1 pili, can interact with microcantilevers functionalized with alpha-D-mannose and can cause shifts in its resonance frequencies. Although E. coli ORN208, which expresses abnormal pili, binds poorly to alpha-D-mannose on the nitrocellulose membrane of a FAST slide, it did cause a detectable shift in resonance frequency when interacting with the alpha-D-mannose functionalized microcantilevers. [DOI: 10.1115/1.4002363]

• 出版日期2011-1
• 单位东北大学