Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae

作者：Shi Shuobo; Choi Yook Wah; Zhao Huimin; Tan Meng How; Ang Ee Lui^*

来源：Bioresource Technology, 2017, 245: 1343-1351.

DOI：10.1016/j.biortech.2017.06.114

• Butanol
• Biosensor
• Transcriptome
• Promoter
• Saccharomyces cerevisiae

The present study aimed to develop a universal methodology for the discovery of biosensors sensitive to particular stresses or metabolites by using a transcriptome analysis, in order to address the need for in vivo biosensors to drive the engineering of microbial cell factories. The method was successfully applied to the discovery of 1-butanol sensors. In particular, the genome-wide transcriptome profiling of S. cerevisiae exposed to three similar short-chain alcohols, 1-butanol, 1-propanol, and ethanol, identified genes that were differentially expressed only under the treatment of 1-butanol. From these candidates, two promoters that responded specifically to 1-butanol were characterized in a dose-dependent manner and were used to distinguish differences in production levels among different 1-butanol producer strains. This strategy opens up new opportunities for the discovery of promoter-based biosensors and can potentially be used to identify biosensors for any metabolite that causes cellular transcriptomic changes.

• 出版日期2017-12
• 单位南阳理工学院