Anethole and eugenol reduce in vitro and in vivo leukocyte migration induced by fMLP, LTB4, and carrageenan

作者：Estevao Silva Camila Fernanda; Kummer Raquel; Fachini Queiroz Fernanda Carolina; Grespan Renata; Nogueira de Melo Gessilda Alcantara; Baroni Silmara; Nakamura Cuman Roberto Kenji; Bersani Amado Ciomar Aparecida^*

来源：Journal of Natural Medicines, 2014, 68(3): 567-575.

DOI：10.1007/s11418-014-0839-7

• Anethole
• Eugenol
• Leukocyte recruitment
• Microcirculation

The aim of this study was to investigate the effect of anethole (AN) and eugenol (EUG) on leukocyte migration using in vitro chemotaxis and in situ microcirculation assays. BALB/c mice were used for the in vitro chemotaxis assay, and Wistar rats for the in situ microcirculation assay. We evaluated (a) the in vitro leukocyte migration in response to chemotactic factors (formyl-methionyl-leucyl-phenylalanine [fMLP] and leukotriene B-4 [LTB4]) and (b) the rolling, adhesion, and migration of leukocytes induced by an injection of carrageenan (100 A mu g/cavity) into the scrotum of the animal. In the in vitro chemotaxis assay, AN and EUG at doses of 1, 3, 9, and 27 A mu g/ml significantly inhibited leukocyte migration when stimulated by the chemotactic agents fMLP and LTB4. In the in situ microcirculation assay, AN at doses of 125 and 250 mg/kg and EUG at a dose of 250 mg/kg significantly decreased the number of leukocytes that rolled, adhered, and migrated to perivascular tissue. The results indicate that AN and EUG exert inhibitory effects on leukocyte migration, highlighting their possible use to diminish excessive leukocyte migration in the inflammatory process.

• 出版日期2014-7