Healthy twin live-birth after ionophore treatment in a case of theophylline-resistant Kartagener syndrome

作者：Ebner T^*; Maurer M; Oppelt P; Mayer R B; Duba H C; Costamoling W; Shebl O

来源：Journal of Assisted Reproduction and Genetics, 2015, 32(6): 873-877.

DOI：10.1007/s10815-015-0486-2

• Ca2+-ionophore
• Kartagener syndrome
• Live birth
• Primary cilia dyskinesis
• Twin pregnancy

To evaluate whether it is a feasible option to target the oocyte (with Ca2+-ionophore) in case that sperm motility cannot be restored in Kartagener syndrome.
A case of a male Kartagener syndrome with exclusively immotile spermatozoa that did not react to the dimethylxanthine theophylline. Thus, half of the associated oocytes were treated for 15 min with the ready-to-use- ionophore CultActive immediately after ICSI whereas the other 50 % were injected with routine ICSI without artificial oocyte activation. Rates of fertilization, blastulation, pregnancy and live birth were evaluated.
Fertilization check revealed that none of the conventionally injected but 4/6 (66.7 %) of the artificially activated oocytes showed two pronuclei. Three embryos were of good and one of fair quality. Corresponding blastocyst formation rate was 3 out of 4 (75 %). A double embryo transfer led to a healthy twin birth in the 34th week of gestation (two boys with a birth weight of 1724 g and 2199 g).
This case indicates that Ca2+-ionophore treatment in cycles from theophylline-resistant Kartagener syndrome patients is a feasible option. The future will show if routine application of A23187 in Kartagener or primary cilia dyskinesis patients will be of benefit.

• 出版日期2015-6