Adaptive changes in serotonin metabolism preserve normal behavior in mice with reduced TPH2 activity

作者：Mosienko Valentina; Matthes Susann; Hirth Natalie; Beis Daniel; Flinders Michael; Bader Michael; Hansson Anita C; Alenina Natalia^*

来源：Neuropharmacology, 2014, 85: 73-80.

DOI：10.1016/j.neuropharm.2014.05.015

• Serotonin
• TPH2
• Gene polymorphism
• Anxiety
• Depression-like behavior
• 5-HT1A receptor

Polymorphisms in the TPH2 gene coding for the serotonin synthesizing enzyme in the brain are considered as risk factors associated with depression and anxiety in humans. However, whether a certain variation in the TPH2 gene leads to decreased brain serotonin production and development of psychological abnormalities remains unresolved.
We generated a new mouse model, carrying one Tph2-null allele and one Tph2(1473)G-allele, coding for a hypoactive form of the enzyme. We tested these mice along with C57BL/6 mice (Tph2(c/c)), congenic C57BL/6 mice homozygous for the Tph2(1473)G-allele (Tph2(G/G)), and heterozygous Tph2-deficient mice (Tph2(c/-)) for anxiety- and depression-like behavior, and evaluated brain serotonin metabolism and 5-HT1AR signaling by high-performance liquid chromatography and quantitative autoradiography, respectively.
Progressive reduction in TPH2 activity had no effect on emotional behavior, and only slightly affected brain serotonin levels. However, serotonin degradation rate was drastically decreased in mice with reduced TPH2 activity, thereby compensating for the lowered rate of serotonin production in these mice. In addition, the hypothermic response to the 5-HT1AR agonist, 8-OH-DPAT, was attenuated in mice with reduced serotonin production. In contrast, 5-HT1A autoreceptor density and G-protein coupling were not changed in mice with gradual decrease in central serotonin.
Taken together, these data suggest that in conditions of reduced serotonin production lowered serotonin degradation rate contributes to the maintenance of brain serotonin at levels sufficient for adequate behavior responses. These findings reveal that decreased TPH2 activity cannot be considered a reliable predisposition factor for impaired emotional behavior.

• 出版日期2014-10
• 单位上海市精神卫生中心