Altering the regioselectivity of cytochrome P450 BM-3 by saturation mutagenesis for the biosynthesis of indirubin

作者：Hu, Sheng; Huang, Jun; Mei, Lehe^*; Yu, Qing; Yao, Shanjing; Jin, Zhihua

来源：Journal of Molecular Catalysis B: Enzymatic , 2010, 67(1-2): 29-35.

DOI：10.1016/j.molcatb.2010.07.001

• Cytochrome P450 BM-3
• Regioselectivity
• Saturation mutagenesis
• Indirubin

Engineering the regioselectivity of enzymes to fulfill application needs is an important goal of protein engineering. To create biocatalysts suitable for the biosynthesis of indirubin (a drug for chronic myelogenous leukemia and a novel promising anticancer agent), cytochrome P450 BM-3 was engineered by site-directed saturation mutagenesis at position D168 to alter its hydroxylation regioselectivity towards indole. One mutant, D168W, was created. It primarily produces indirubin (similar to 90%) whereas the parent enzyme primarily forms indigo (similar to 85%). Docking calculations showed that the mutation altered the orientation of indole, and that the C-2 of the indole pyrrole ring was closer to the heme iron of P450 BM-3 than the C-3. The mutation possibly shifted the hydroxylation preference of P450 BM-3 for indole from the C-3 to C-2, which may be responsible for the reversal of distribution of the product yield. This mutant yielded high-purity indirubin and may be a good starting point for the biosynthesis of indirubin.

• 出版日期2010-11
• 单位浙江科技学院; 浙江大学; 浙江大学宁波理工学院