Use of an IL1-receptor antagonist to prevent the progression of tendinopathy in a rat model

作者：Berkoff David J^*; Kallianos Steven A; Eskildsen Scott M; Weinhold Paul S

来源：Journal of Orthopaedic Research, 2016, 34(4): 616-622.

DOI：10.1002/jor.23057

• inflammatory cytokine
• patellar tendon
• IL1
• carrageenan
• tendon inflammation

This study evaluated if inhibiting IL1- activity with an IL1-receptor antagonist (IL1-RA) will prevent pathologic changes commonly seen in tendinopathy. Thirty-six Sprague-Dawley retired-breeder rats were divided into three groups having weekly bilateral patellar tendon injections: CON (0.1ml Saline), CAR (0.1ml 2% carrageenan), IL1-RA (0.1ml 2% CAR plus 0.94mg of the IL1-RA, 2.5mg/kg). Carrageenan was used to establish tendinopathy in two groups due to its ability to develop tendinopathy in prior studies. Animals were euthanized 3 weeks after initial injection. The CAR group demonstrated significantly (p<0.05) shorter tendon lengths (8.61 +/- 0.38mm) relative to CON (8.94 +/- 0.38mm) that was prevented in the IL1-RA (9.02 +/- 0.30mm) as well as significantly increased collagenase activity in the CAR (0.061 +/- 0.043) compared to CON (0.027 +/- 0.015) (p<0.05). By histological evaluation, the CAR group demonstrated significantly greater inflammation than IL1-RA, and CON (p<0.05). CAR showed a trend for increased cross-sectional area relative to CON that was absent in the IL1-RA. IL1-RA can effectively inhibit the development of mechanical, chemical, and histologic changes seen with carrageenan-induced tendonitis.

• 出版日期2016-4