Aberrant Expression miR-196a Is Associated With Abnormal Apoptosis, Invasion, and Proliferation of Pancreatic Cancer Cells

作者：Liu Minghao; Du Yiqi; Gao Jun; Liu Jianqiang; Kong Xiangyu; Gong Yanfang; Li Zhaoshen^*; Wu Hongyu; Chen Hui

来源：Pancreas, 2013, 42(7): 1169-1181.

• microRNA
• miR-196a
• ING5
• pancreatic cancer

Objectives: MiR-196a levels inversely correlated with survival in pancreatic adenocarcinoma patients. However, the functional contributions of miR-196a to pancreatic cancer remain unclear. %26lt;br%26gt;Methods: Three lentiviral vectors encoding microRNA miR-196a precursor, inhibitor, and scrambled microRNA oligomer were transfected into Panc-1 cells, respectively. Then we explored the regulation of inhibitor of growth 5 (ING5) expression by miR-196a and its impact on apoptosis, invasion, and growth of pancreatic cancer cells. The lentiviral transfected Panc-1 cells were surgically implanted into the pancreas of mice. In vivo tumor growth and ING5 expression were measured. %26lt;br%26gt;Results: Down-regulation of ING5 expression was detected in cells transfected with miR-196a precursor (P %26lt; 0.01), accompanied by less apoptosis, increased invasion, and proliferation compared with control cells (P %26lt; 0.05). Cells transfected with miR-196a inhibitor revealed an opposite trend. Smaller detectable tumors were found in only 60% of mice after implantation of Lenti. miR-196a inhibitor-transfected Panc-1 cells compared with controls (360.7 +/- 303.6 mm(3) vs 511.58 +/- 365.9 mm3 in controls; P %26lt; 0.01). %26lt;br%26gt;Conclusion: Our results provide experimental evidence to support aberrant expression of miR-196a is associated with abnormal apoptosis, invasion, and proliferation of pancreatic cancer cells.

• 出版日期2013-10
• 单位中国人民解放军第二军医大学