A novel lysosome-targeted fluorogenic probe based on 5-triazole-quinoline for the rapid detection of hydrogen sulfide in living cells

作者：Dou, Yandong; Gu, Xiaoxu; Ying, Shasha; Zhu, Shen; Yu, Shian; Shen, Wei^*; Zhu, Qing^*

来源：Organic and Biomolecular Chemistry, 2018, 16(5): 712-716.

DOI：10.1039/c7ob02881d

• 2-PHOTON FLUORESCENT-PROBE
• LIVE CELLS
• H2S
• MITOCHONDRIA
• DISEASE
• SULFUR
• BRAIN

A fluorogenic probe based on the novel fluorophore 5-triazolequinoline was developed for the detection of hydrogen sulfide, an endogenous signaling molecule associated with the development of various diseases. The lysosome-targeted probe Lyso-HS was synthesized via C-H direct azidation from 8-aminoquinoline; it was able to detect H2S in 1 min and exhibited excellent turn-on ability with 95-fold fluorescence enhancement based on a new fluorochrome. The high quenching efficiency was further verified using time-dependent density functional theory (TDDFT). The probe also exhibited high selectivity and a low detection limit (as low as 214.5 nM), which has practical applications for disease detection and monitoring.

• 出版日期2018-2-7
• 单位浙江工业大学