A TAF4 coactivator function for E proteins that involves enhanced TFIID binding

作者：Chen Wei Yi; Zhang Jinsong; Geng Huimin; Du Zhimei; Nakadai Tomoyoshi; Roeder Robert G^*

来源：Genes & Development, 2013, 27(14): 1596-1609.

DOI：10.1101/gad.216192.113

• TAF4
• TAFH
• TFIID
• transcription coactivator
• E proteins
• E2A
• HEB

The multisubunit TFIID plays a direct role in transcription initiation by binding to core promoter elements and directing preinitiation complex assembly. Although TFIID may also function as a coactivator through direct interactions with promoter-bound activators, mechanistic aspects of this poorly defined function remain unclear. Here, biochemical studies show a direct TFIID-E-protein interaction that (1) is mediated through interaction of a novel E-protein activation domain (activation domain 3 [AD3]) with the TAF homology (TAFH) domain of TAF4, (2) is critical for activation of a natural target gene by an E protein, and (3) mechanistically acts by enhancing TFIID binding to the core promoter. Complementary assays establish a gene-specific role for the TAFH domain in TFIID recruitment and activation of a large subset of genes in vivo. These results firmly establish TAF4 as a bona fide E-protein coactivator as well as a mechanism involving facilitated TFIID binding through direct interaction with an E-protein activation domain.

• 出版日期2013-7-15