摘要

A sensitive and specific liquid chromatography-electrospray ionization-tandem mass spectrometry method has been developed and validated for the quantification of huperzine A in human plasma. After the addition of trimetazidine, the internal standard (IS) and sodium hydroxide, plasma samples were extracted using 5 mL ethyl acetate. The compounds were separated on an Agilent Zorbax SB C-18 column (100 mm x 2.1 mm ID, dp 3.5 mu m) using an elution system of 10 mM ammonium acetate solution-methanol-formic acid (18:82:0.1, v/v) as the mobile phase. The quantification of target compounds was obtained by using multiple reaction monitoring (MRM) transitions: m/z 243.1, 210.1 and 267.2, 166.0 were measured in positive mode for huperzine A and IS. Linearity was established for the range of concentrations 0.01-4.0 ng mL(-1) with a coefficient of correlation (r) of 0.9991. The lower limit of quantification (LLOQ) was identifiable and reproducible at 0.01 ng mL(-1). The method has been successfully applied to study the pharmacokinetics of huperzine A in healthy male Chinese volunteers.