Up-regulation of HIV-1 transduction in nondividing cells by double-strand DNA break-inducing agents

作者：Smith Johanna A; Daniel Rene^*

来源：Biotechnology Letters, 2011, 33(2): 243-252.

DOI：10.1007/s10529-010-0449-8

• Gene therapy
• HIV
• Vector
• DNA damage

Efficient HIV-1 transduction depends on a number of cellular co-factors. Cellular double-strand DNA break (DSB) repair proteins have been proposed, by ourselves and others, to be required for efficient HIV-1 transduction. Expression and/or activity of these DNA repair proteins can be induced by the introduction of DSBs into the host cell genome. HIV-1 transduction was up-regulated by treatment with DSB-inducing agents in both drug-arrested cells and differentiated neuronal cells. The presented data support the hypothesis that DSB repair proteins are involved in the early steps of the retroviral life-cycle.

• 出版日期2011-2