Automatic Biological Cell Counting Using a Modified Gradient Hough Transform

作者:Denimal Emmanuel*; Marin Ambroise; Guyot Stephane; Journaux Ludovic; Molin Paul
来源:Microscopy and Microanalysis, 2017, 23(1): 11-21.
DOI:10.1017/S1431927616012617

摘要

We present a computational method for pseudo-circular object detection and quantitative characterization in digital images, using the gradient accumulation matrix as a basic tool. This Gradient Accumulation Transform (GAT) was first introduced in 1992 by Kierkegaard and recently used by Kaytanli & Valentine. In the present article, we modify the approach by using the phase coding studied by Cicconet, and by adding a local contributor list (LCL) as well as a used contributor matrix (UCM), which allow for accurate peak detection and exploitation. These changes help make the GAT algorithm a robust and precise method to automatically detect pseudo-circular objects in a microscopic image. We then present an application of the method to cell counting in microbiological images.

  • 出版日期2017-2