Fasciola hepatica: Comparison of the sedimentation and FLOTAC techniques for the detection and quantification of faecal egg counts in rats

作者：Duthaler Urs; Rinaldi Laura; Maurelli Maria P; Vargas Mireille; Utzinger Juerg; Cringoli Giuseppe; Keiser Jennifer^*

来源：Experimental Parasitology, 2010, 126(2): 161-166.

DOI：10.1016/j.exppara.2010.04.020

• Fasciola hepatica
• Diagnosis
• Sedimentation
• FLOTAC
• Sensitivity
• Faecal egg count
• Efficiency

We compared the sedimentation and FLOTAC techniques for the detection and quantification of Fasciola hepatica eggs in faecal samples obtained from 120 experimentally-infected rats before intervention, and in 42 rats after drug administration. Additionally, the average time for a single test was determined. A single FLOTAC showed a higher sensitivity (92.6%) than 2, 4 and 8 sedimentation readings (63.0-85.2%) for detecting F. hepatica eggs in rat faeces post-treatment. On average, it took 21 min to prepare and examine a single FLOTAC, whereas 114 min were needed for the sedimentation method including the reading of 8 slides. In both treated and untreated rats, the sedimentation method resulted in higher mean faecal egg counts (FECs) than FLOTAC (P < 0.05). In view of the high sensitivity and efficiency, the FLOTAC technique holds promise for experimental work in the F. hepatica-rat model. Additional research is needed to determine the reasons for the observed differences in FECs.

• 出版日期2010-10