A simple light-emitted diode-induced fluorescence detector using optical fibers and a charged coupled device for direct and indirect capillary electrophoresis methods

作者：Arraez Roman D; Fernandez Sanchez JF; Cortacero Ramirez S; Segura Carretero A^*; Fernandez Gutierrez A

来源：Electrophoresis, 2006, 27(9): 1776-1783.

DOI：10.1002/elps.200500701

• capillary electrophoresis
• derivatized fluorescence detection
• indirect fluorescence detection
• light-emitted diode-induced fluorescence
• native fluorescence detection

We constructed a simple fluorescence detector for both direct and indirect CE methods using a blue light-emitted diode (470 nm) as excitation source, a bifurcated optical fiber as a waveguide, and a CCD camera as a detector. The connection of all the components is fairly easy even for nonexperts and the use of a CCD camera improves the applicability of this detector compared to the others using PMTs because it permits the recording of 2-D electropherograms or phosphorescence measurements. This detector provides a compact, low cost, and rapid system for the determination of native fluorescence compounds which have high quantum yields by CE with direct fluorescence detection, showing an LOD of 2.6 x 10(-6) M for fluorescein; the determination of fluorescence derivative compounds by CE with direct fluorescence detection, showing an LOD of 1.6 x 10(-7) IM for FITC-labeled 1,6-diaminohexane; and non-fluorescence compounds by CE with indirect fluorescence detection with an LOD of 2.7 x 10(-6) M for gallic acid.

• 出版日期2006-5