Determination of coenzyme Q(10) in human breast milk high-performance liquid chromatography

作者：Tang Peter H^*; Miles Michael V; Steele Paul; Davidson Barbara S; Geraghty Sheela R; Morrow Ardythe L

来源：Biomedical Chromatography, 2006, 20(12): 1336-1343.

DOI：10.1002/bmc.702

• coenzyme Q(10)
• breast milk
• HPLC
• electrochemical detection

An isocratic HPLC method was developed for the determination of coenzyme Q(10) (CoQ(10)) in human breast milk. After a single-step liquid-liquid extraction, the milk extract was injected directly into the HPLC system. The analytical method is based on pre-column inline treatment of CoQ(10). Chromatographic separation of CoQ, and coenzyme 0, (CoQ,) internal standard was achieved using a reversed-phase Microsorb-MV C-18 analytical column. CoQ(10) and CoQ(9) were monitored by an electrochemical detector (ECD). An excellent linearity (r=0.999) was observed for CoQ(10) in the concentration range 0.06-2.5 mu mol L-1 in breast milk. The limit of quantitation (LOQ) was 60 nmol L-1. Coefficients of variations (CVs) for intra-day and inter-day assay precisions were less than 5%. A total of 194 breast milk samples were analyzed for the CoQ(10) concentration; the mean value was 0.32 +/- 0.21 mu mol L-1.

• 出版日期2006-12