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摘要
Multiphoton microscopy allows imaging of cellular activity in living tissue explants and in whole animals and has thus become a powerful tool for studying neuronal activity. The miniaturization of laser-scanning microscopy promises to extend activity measurements to awake, freely moving animals. Here we describe and evaluate a miniature multiphoton microscope based on a "piezolever fiber scanner" (PLFS). Our current PLFS has a scan range of 1.1 rum and a resonance frequency of 790 Hz. It allows, unlike resonance-based designs, a lateral shift of the scanned area, and offers the prospect of random-access (vector) scanning.
- 出版日期2007-8-1
- 单位河北医科大学
